2. AM hyperinsulinemic-euglycemic clamp data.

A vertical line at 120 min marks the end of the Ins2h-A and the start of the Ins2h-B clamp period. Arterial plasma glucose (A) and insulin (B) concentrations, as well as glucose infusion rates (D) required to maintain euglycemia over the course of the 4h morning period, are shown for Ins4h, Ins2h-A, and Ins2h-B; n=6/group. Bar graphs indicate the area under the curve (AUC) for arterial insulin concentrations (C) and glucose infusion rates (E) during the 4h morning period in each dog. Data are expressed as mean ± SEM. *P<0.05, **P<0.01 between groups. All comparisons that did not reach statistical significance are denoted ns.