Table 2:
PM clamp non-esterified fatty acid and metabolite flux data.
| Parameter and group | Non-Clamp | PM Clamp | ||||
|---|---|---|---|---|---|---|
| 360 | 420 | 480 | 540 | 600 | PM Clamp Mean | |
| Arterial Plasma NEFA (μmol/L) | ||||||
| Ins4h | 828±109 | 113±37 | 77±30 | 67±22 | 46±6 | 80±17 |
| Ins2h-A | 960±108 | 165±44 | 83±28 | 71±22 | 56±19 | 128±40 |
| Ins2h-B | 1127±140 | 102±23 | 86±18 | 50±3 | 86±15* | 100±17 |
| Net Hepatic NEFA Uptake (μmol/kg/min) | ||||||
| Ins4h | 2.1±0.3 | 0.4±0.2 | 0.4±0.2 | 0.2±0.1 | 0.3±0.1 | 0.3±0.1 |
| Ins2h-A | 1.9±0.2 | 0.5±0.1# | 0.2±0.1 | 0.3±0.2 | 0.4±0.1 | 0.4±0.1 |
| Ins2h-B | 2.1±0.4 | 0.3±0.1 | 0.2±0.1 | 0.1±0.1 | 0.2±0.1 | 0.2±0.1 |
| Arterial Blood Glycerol (μmol/L) | ||||||
| Ins4h | 88±12 | 26±5 | 22±2 | 19±4 | 17±4 | 23±3 |
| Ins2h-A | 92±6 | 37±5 | 33±6 | 27±5 | 26±5 | 32±5 |
| Ins2h-B | 123±15 | 28±5 | 25±5 | 29±7 | 21±4 | 27±5 |
| Net Hepatic Glycerol Uptake (μmol/kg/min) | ||||||
| Ins4h | 2.0±0.4 | 0.4±0.1 | 0.3±0.1 | 0.2±0.1* | 0.2±0.1 | 0.3±0.1 |
| Ins2h-A | 2.0±0.2 # | 0.8±0.1* | 0.6±0.1 | 0.6±0.1 | 0.6±0.1* | 0.6±0.1 |
| Ins2h-B | 2.8±0.3 | 0.4±0.1 | 0.4±0.1 | 0.6±0.1 | 0.3±0.1# | 0.5±0.1 |
| Arterial Blood Lactate (μmol/L) | ||||||
| Ins4h | 659±169 | 781±55 | 656±85 | 770±103 | 841±92 | 813±77 |
| Ins2h-A | 323±25 | 848±94 | 673±84 | 798±82 | 824±75 | 776±74 |
| Ins2h-B | 380±40 | 796±82 | 658±72 | 710±50 | 753±68 | 711±45 |
| Net Hepatic Lactate Output (μmol/kg/min) | ||||||
| Ins4h | −2.8±2.8 | 16.0±1.8 | 10.4±2.4 | 11.6±2.6 | 10.7±2.4 | 12.3±2.1 |
| Ins2h-A | −3.2±0.8 | 14.2±3.3 | 7.9±2.3 | 7.0±2.0 | 6.7±1.9 | 9.8±2.4 |
| Ins2h-B | −1.1±1.8 | 15.8±2.5 | 9.9±1.7 | 9.7±1.7 | 8.8±2.4 | 11.3±1.8 |
| Arterial Blood Alanine (μmol/L) | ||||||
| Ins4h | 265±39 | 282±25 | 246±19 | 234±15 | 220±17 | 250±20 |
| Ins2h-A | 224±15 | 276±18 | 244±27 | 233±30 | 221±29 | 245±27 |
| Ins2h-B | 223±10 | 243±15 | 238±19 | 278±55 | 217±15 | 238±14 |
| Net Hepatic Alanine Uptake (μmol/kg/min) | ||||||
| Ins4h | 2.4±0.5 | 2.1±0.2 | 1.8±0.1 | 1.8±0.2 | 1.7±0.3 | 2.0±0.2 |
| Ins2h-A | 1.8±0.2 | 1.3±0.2* | 1.7±0.2 | 1.6±0.1 | 1.5±0.2 | 1.4±0.1 |
| Ins2h-B | 1.8±0.3 | 1.5±0.2 | 1.4±0.1 | 2.2±0.5 | 1.1±0.4 | 1.5±0.1 |
The non-clamp period was from 240 to 360 min, and the PM clamp period was from 360 to 600 min. Average group values are presented at the onset of the clamp (360 min) and every hour during the PM clamp. Blood samples were collected every 30 min during the non-clamp and PM clamp periods. Data are represented as mean ± SEM, n=6 per group.
*
P<0.05 for Ins4h vs. Ins2h-A and Ins4h vs. Ins2h-B,
#
P<0.05 for Ins2h-A vs. Ins2h-B.