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Phospho-l- and phospho-d-serine both inhibit E. histolytica ingestion of calcium-treated erythrocytes. Phospho-d-serine, phospho-l-serine, phospho-l-threonine, and dl-serine were added to M199S medium and incubated with E. histolytica for 30 min prior the performance of a phagocytosis assay. Erythrocytes were added at a 1:5 ratio (amebae to erythrocytes) and incubated at 37°C for 20 min. Amebae were fixed, counted, and scored for phagocytosis. Data are represented as means ± SD (n = 3).
