FIG. 6.
MCP-1 chemokine production of CD18 hypomorph (CD18hypo) versus wild-type DCs. MCP-1 secretion, determined by ELISA, by bone marrow-derived DCs from wild-type (open circles) and CD18 hypomorph (open squares) mice at increasing ratios of B. burgdorferi to DCs. Each symbol corresponds to dendritic cells originating from the bone marrow of one mouse, with the average of each experimental group depicted as a horizontal line. Data from three independent experiments were combined. The difference between values for WT and CD18 hypomorph mice was statistically significant at all tested ratios (P < 0.05). The differences were also statistically significant when the nonparametric Mann-Whitney U test was used. Average MCP-1 values for B. burgdorferi cell:DC ratios of 0:1, 0.1:1, 1:1, 10:1, and 100:1 were 281.9, 299.4, 267.4, 442.4, and 521 for WT mice, respectively, and 669.2, 710, 600.4, 874, and 1060.4 for CD18 hypomorph mice, respectively. Bb, B. burgdorferi.