Table 2.
Primer name | Sequence (5ʹ—3ʹ) |
---|---|
chloPTPN1-HA-F_EcoRI | CCGAATTCATGGAGATGGAAAAGGAG |
chloPTPN1-HA-R_KpnI | CGGGTACCCTAAGCGTAATCTGGAACATCGTATGGGTATGTGTTGCTGTTGAACA |
chloPTPN1-myc-R_KpnI | CGGGTACCCTACAGATCCTCTTCTGAGATGAGTTTTTGTTCTGTGTTGCTGTTGAACA |
chloPTPN1_D181A-F | TTCCACTATACCACATGGCCCGCCTTTGGAGTCCCCGAATCGCCA |
chloPTPN1_D181A-R | TGGCGATTCGGGGACTCCAAAGGCGGGCCATGTGGTATAGTGGAA |
chloPTPN1_C215S-F | CACGGGCCCGTCGTGGTGCACTCCAGTGCGGGTATCGGCAGGTCT |
chloPTPN1_C215S-R | AGACCTGCCGATACCCGCACTGGAGTGCACCACGACGGGCCCGTG |
chloPTPN1-F_EcoRI | CGGAATTCATGGAGATGGAAAAGGAGTTCGAGCAG |
chloPTPN1-R_SalI | GTGTCGACTGTGTTGCTGTTGAACAGGAACCTG |
chlo refers to Chlorocebus sabaeus.