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. 2024 May 23;55:67. doi: 10.1186/s13567-024-01316-8

Table 2.

Primers used for plasmid construction

Primer name Sequence (5ʹ—3ʹ)
chloPTPN1-HA-F_EcoRI CCGAATTCATGGAGATGGAAAAGGAG
chloPTPN1-HA-R_KpnI CGGGTACCCTAAGCGTAATCTGGAACATCGTATGGGTATGTGTTGCTGTTGAACA
chloPTPN1-myc-R_KpnI CGGGTACCCTACAGATCCTCTTCTGAGATGAGTTTTTGTTCTGTGTTGCTGTTGAACA
chloPTPN1_D181A-F TTCCACTATACCACATGGCCCGCCTTTGGAGTCCCCGAATCGCCA
chloPTPN1_D181A-R TGGCGATTCGGGGACTCCAAAGGCGGGCCATGTGGTATAGTGGAA
chloPTPN1_C215S-F CACGGGCCCGTCGTGGTGCACTCCAGTGCGGGTATCGGCAGGTCT
chloPTPN1_C215S-R AGACCTGCCGATACCCGCACTGGAGTGCACCACGACGGGCCCGTG
chloPTPN1-F_EcoRI CGGAATTCATGGAGATGGAAAAGGAGTTCGAGCAG
chloPTPN1-R_SalI GTGTCGACTGTGTTGCTGTTGAACAGGAACCTG

chlo refers to Chlorocebus sabaeus.