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Purification and analysis of the GFPmut3-CTA2/CTB (GFP-CT) chimera. (A) SDS-PAGE. Lane 1, d-galactose eluate 1 from initial preparation; lane 2, d-galactose eluate 2 from initial preparation; lane 3, d-galactose flowthrough; lane 4, d-galactose eluate 1; lane 5, d-galactose eluate 2 from repurification after incubation at 4°C for greater than 1 month. (B) Anti-CTA (α-CTA)/α-CTB and α-GFP Western blots of eluates 1 and 2 described in panel A.
