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Development of LTI chimeras. (A) Plasmid pJKT68 constructed for transport of LTI chimeras to the periplasm of E. coli through the tat secretion system. Asterisk, coding region for amino acids 162 to 240 of LTI. (B) SDS-PAGE of the mRFP-LTI chimera after purification on d-galactose agarose. Lane 1, flowthrough; lane 2, eluate 1; and lane 3, eluate 2. (C) Binding and internalization of the mRFP-LTI chimera (50 μg/ml) on Vero cells at 4°C (frame 1) or 37°C (frame 2) for 1 h.
