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Development of LTIIb chimeras. (A) Plasmid pJKT66 constructed for the expression and transport of LTIIb chimeras to the periplasm of E. coli through the tat secretion system. Asterisk, amino acids 158 to 243. (B) Binding and internalization of the GFP-LTIIb periplasmic extract on Vero cells at 4°C (frame 1) and 37°C (frame 2) for 1 h or of the RFP-LTIIb chimera at 4°C (frame 3) and 37°C (frame 4) for 1 h.
