TABLE 1.
Inflammatory parameters in BALF of C57BL/6 mice sequentially receiving Ad vectors and S. aureusa
| Treatment | No. of mice | Content (range)
|
||
|---|---|---|---|---|
| BALF protein (μg/ml) | BALF MPO (arbitrary units) | BALF TNF (pg/ml) | ||
| PBS-PBS | 3 | 314.7 (232.7-320.4) | 0.031 (0.008-0.26) | 15.0 (3.75-48.75) |
| Ad-LacZ-S. aureus | 6 | 743.2 (579.3-851.6) | 0.094 (0.049-0.299) | 2,283.3* (975.0-3,225.0) |
| Ad-elafin-S. aureus | 8 | 567.0* (433.7-689.3) | 0.059 (0.027-0.165) | 577.5*† (221.9-1,188.1) |
a
Female C57BL/6 mice received sequential intratracheal treatments of Ad-LacZ or Ad-elafin and then S. aureus as described in Materials and Methods. At 24 h after bacterial administration, BALF was obtained and centrifuged; the cell pellet was used for differential cell analysis, whereas supernatants were used for the determination of total protein and TNF levels and MPO activity (given as median values). *, significantly different from the PBS-PBS group; †, significantly different from the Ad-LacZ-S. aureus group (Mann-Whitney, P < 0.05).