Table 2.
Serum (%) | Cells2 (×106) | T Antigen | %G1 |
2.5 | 3.99 | 60.4 | 71.1 |
5 | 4.02 | 57.6 | 73.7 |
10 | 3.62 | 57.1 | 71.8 |
1 The P0-3DtkT#5 cell line was inoculated at 106 per 10 cm plate in DMEM containing the indicated amount of serum. Cells were harvested after 3 days and analysed for Tag level and %G1 by cytometry. 2Cell number = final cell number per 10 cm dish.