Table 4.
Summary statistics from enriching 100 human fecal DNA samples with two library preparation methods using CryptoCap_100k baits and obtaining Illumina PE150 reads. Source tables are Tables S17 and S18.
| iTru Libraries | iNextEra Libraries | |||||
|---|---|---|---|---|---|---|
| Average values | Not enriched | Single enriched | Not enriched | Single enriched | Double enriched | |
| % retained after quality filtering | 61.53 | 83.87 | 89.70 | 90.79 | 94.48 | |
| % reads on target C. parvum | 0.04 | 35.62 | 0.033 | 15.85 | 64.98 | |
| % reads on target 10 genomes | 0.11 | 41.23 | 0.100 | 17.90 | 65.78 | |
| Enrichment factor * | N/A | 2,095 | N/A | 1,112 | 10,700** | |
| Depth per 100k * | 0.00 | 1.12 | 0.001 | 0.563 | 2.702 | |
| Breadth per 100k * | 0.00 | 2.25 | 0.065 | 1.79 | 5.99 | |
| Number of samples | 73 | 100 | 91 | 91 | 91 | |
*
(matching C. parvum)
**
This value corresponds to the enrichment factor of double-enriched samples compared to not-enriched counterparts, which is not displayed in Tables S17 and S18.