FIG. 3.

Sepharose 4B gel filtration of ³H-labeled CsCl mucin degraded by E. histolytica-secreted products. (A) Purified mucin (3 × 10⁵ counts per minute) was incubated in PBS alone or with 200 μg of secreted products at 37°C for 18 h. The digests were separated by gel filtration, and aliquots of each fraction were analyzed by liquid scintillation counting. The column was calibrated with blue dextran (BD, 2,000 kDa; Pharmacia, Uppsala, Sweden) and bovine serum albumin (BSA, 68 kDa; Sigma-Aldrich). (B and C) CsCl density gradient centrifugation of ³H-labeled mucin incubated in PBS (B) or amoeba-secreted components (C). Data represent the results of one experiment repeated twice with similar results.