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Cytokine mRNA levels in sequential skin biopsies from patients with T1R and nonreactional patients before and during treatment. Levels of cytokine mRNA for TNF-α, IL-1β, TGF-β1, IL-10, and HPRT1 in skin biopsies were quantified using real-time PCR assays. The results are expressed as the ratio of cytokine mRNA to HPRT1 mRNA. Each dot represents a patient. Significant differences compared with zero time are indicated as follows: one asterisk, P < 0.05; and two asterisks, P < 0.01.
