Table 1. Effect of salt on the kinetic parameters of bicarbonate dehydration activity of dCA II.

[NaCl], M	Km,* mM	Kcat,* (ms)-1	Kenz, (M·μs)-1
dCA II
0	28.7 ± 3.0	38.3 ± 1.5	1.33
0.1	30.1 ± 4.5	44.6 ± 2.5	1.48
0.25	32.2 ± 7.9	41.2 ± 4.0	1.27
0.5	33.4 ± 12.4	39.1 ± 5.9	1.17
hCA I
0	19.7 ± 2.5	29.5 ± 1.3	1.50
0.1	80.1 ± 11.4	28.7 ± 2.3	0.36

Steady-state kinetics parameters were determined essentially as described in ref. 18. Assay mixtures contained the indicated concentrations of NaCl and 0.28 μM hCA I or 0.54 μM dCA II.

^*Values are ± SE.