Figure 5.

Effect of B355252 on astrocyte activation detected by GFAP immunofluorescent labeling: (A) representative images of GFAP labeling (GFAP-positive astrocytes are labeled in green and nuclei in blue by DAPI); (B) number of astrocytes in each experimental group; (C) mean number of dendrites per cell; (D) mean percentage of GFAP positively labeled area in each group. LPS markedly increased the number of astrocytes, the number of dendrites per astrocyte, and the percentage of GFAP positively labeled area. B355252 profoundly reduced these counts in the cortex and Cpu. Data in the bar graphs are presented as mean +/− SD. Significance levels are denoted as *, ** p < 0.05, 0.01 vs. NC (naïve control); #, ## for p < 0.05, 0.01 vs. LPS24h.