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. 2024 May 23;17:27. doi: 10.1186/s13041-024-01101-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Hierarchical cluster analysis to categorize eNAc regions by Scratch-Fos correlations under different itch/scratch conditions. A hierarchical cluster analysis to categorize eNAc regions by scratching and Fos expression in four itch/scratch conditions. A Left: A dendrogram representing clusters based on the Scratch-Fos correlation coefficients of 95 regions (4 zones × 8 sectors × 3 planes, excluding zone 4-sector 5 in the rostral slice). The vertical dashed line indicates the Euclidean distance threshold used to separate the regions into 5 independent clusters. The three-digit numbers to the right of the dendrogram correspond to plane-zone-sector IDs (Table 1). Right: Heat map plot showing the Scratch-Fos correlation coefficient in each region under four itch/scratch conditions. The color is according to the scale on the right. B The mean Scratch-Fos correlation coefficient in each cluster under the different itch/scratch conditions. The numbers below the figure indicate the cluster numbers shown in Fig. 8A. C Zone-and-sector plots showing the clusters to which each region belongs. Cluster numbers are shown with a color index on the right