Figure 5.

DNA damage of neuron cells in the rat brain determined by immunofluorescence staining for NeuN and cleaved caspase-3. (A) Representative micrographs of immunofluorescence staining are shown for the four groups. The white arrows indicate cells with positive double staining, showing co-expression of NeuN and cleaved caspase-3. (B) The number of neuronal cells with DNA damage from immunofluorescence staining in the four groups was measured. All values are mean ± SEM (n = 12). * p < 0.05 and ** p < 0.01 when compared with the SAH group.