Figure 7.

ERβ agonist conjugates promote skin injury healing. (A) Frostbite skin injury mouse model. (B, C) Skin closure 1 month after frostbite injury under treatment with vehicle, JW-127, or JW-191, and quantification. (D) H&E staining of skin recovered from frostbite injury under treatments. Scale bar, 50 μm. (E, F) Immunostaining of skin slices for Ki67 and p63, with quantification. Scale bar, 50 μm ∗P < 0.05. ∗∗P < 0.01, n = 2, 4, 4. (G) Gene expression in cultured basal skin cells after lentiviral ERβ knockdown using an shRNA. (H) Immunostaining of skin basal cells with Ki67 upon knockdown, with quantification (I). Scale bar, 100 μm ∗∗P < 0.01, n = 3. All values are presented as the mean ± SEM.