FIGURE 4.

Magnitude of HIV/Gag-specific IFN-γ–secreting T lymphocytes following rAd5-EAT-2 covaccination of Ad5 preimmune animals. Ad5 preimmune BALB/c mice (n = 7) were subjected to a homologous prime-boost rAd5 covaccination regimen. At week 34, animals were terminally sacrificed, and splenocytes were harvested and stimulated ex vivo with the 15-mer HIV/Gag-derived immunogenic peptides AMQMLKETI, and IFN-γ ELISPOT assays were completed. Bars represent means ± SD. Statistical analysis was completed using two-way ANOVA with a Bonferroni post hoc test. A p value < 0.05 was deemed statistically significant.