Skip to main content
. Author manuscript; available in PMC: 2024 May 24.
Published in final edited form as: J Immunol. 2012 Jun 27;189(3):1349–1359. doi: 10.4049/jimmunol.1200736

FIGURE 6.

FIGURE 6.

EAT-2 expression increases the magnitude of Gag-specific TCM and TEM CD8+ cell responses in Ad5 immune animals. Magnitude and phenotype analysis of HIV/Gag-specific CD8+ T cells elicited by rAd5-HIV/Gag and rAd5-EAT-2 vaccination regimen were determined in peripheral blood and splenocytes of Ad5 immune mice by multiparameter tetramer-binding assays. (A) Frequency of Gag-specific tetramer-positive TCM (CD62L+CD127+) CD8+ cells 2 wk following the prime vaccination with 1 × 108 vps is shown. (B) Frequency of Gag-specific tetramer-positive TEM (CD62L−CD127+) CD8+ cells 2 wk following the prime vaccination with 1 × 108 vps is shown. (C) Frequency of Gag-specific tetramer-positive TEM (CD62L−CD127+) CD8+ cells in PBMCs 34 wk following the homologous prime-boost vaccination with 1 × 109 vps is shown. (D) Frequency of Gag-specific tetramer-positive TEM CD8+ cells in splenocytes 34 wk following the homologous prime-boost vaccination with 1 × 109 vps is shown. The bars represent means ± SD. Statistical analysis was completed using one-way ANOVA with a Student–Newman–Keuls post hoc test. A p value < 0.05 was deemed statistically significant. *p < 0.05 versus naive animals.