Figure 8.

Effect of MSC^IL-10 treatment on early alloimmune activation 3 days after transplantation. Early alloimmune response was determined using mixed lymphocyte reaction. Donor spleen cells were used as stimulators, and recipient spleen cells, procured 3 days after transplantation, were used as responders. CD4⁺ cell (A) and CD8⁺ cell (B) proliferation with different stimulators to responder cell ratios. Representative cell proliferation results of CD4 and CD8 cells with 1:1 stimulator: recipient cell ratio and eFluor 450 proliferation dye (C). Data mean ± standard deviation and analyzed by 1-way ANOVA with Dunnett’s correction comparing treatment groups to the control group. * p < 0.05, ** p < 0.01. MSC, mesenchymal stromal cell.