FIG. 3.

Formation of reaction product A is dependent upon both time and protein concentration (panel A) and membrane associated (panel B). Panel A shows the formation of reaction product A assayed under standard conditions described in Materials and Methods using 5 μM 1-dephosphorylated Kdo₂-[4′-³²P]lipid A. Assays were carried out over a 2-h time course using H. pylori J99 membranes at the indicated concentrations. Reaction products were separated by TLC and subjected to PhosphorImager analysis. In panel B, crude extract, double-spun cytosol, or washed membranes at a protein concentration of 1 mg/ml were assayed as described for panel A for formation of reaction product A.