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Time course of hydrolysis at pH 4.5 of 1-dephosphorylated Kdo2-[4′-32P]lipid A (panel A) and the in vitro reaction product A (panel B). Reaction product A or the initial substrate, 1-dephosphorylated Kdo2-[4′-32P]lipid A, was subjected to chemical hydrolysis at pH 4.5 (100°C) as described in Materials and Methods for the indicated times. Hydrolysis products were separated by TLC and subjected to PhosphorImager analysis.
