FIG. 5.

MALDI-TOF mass spectrometry of the Kdo hydrolase reaction product (product A) generated from 1-dephosphorylated Kdo₂-lipid A. The reaction product generated from incubation of 1-dephosphorylated Kdo₂-lipid A with H. pylori J99 membranes was purified by DEAE cellulose chromatography as described in Materials and Methods. Panel A shows the spectra of the starting 1-dephosphorylated Kdo₂-lipid A substrate, and panel B shows the resulting reaction product containing a single Kdo sugar, 1-dephosphorylated Kdo-lipid A. Both spectra were acquired in the negative-ion mode. MW, molecular weight.