TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| H. pylori strains | ||
| J99 | Wild type | ATCC 700824 |
| 26695 | Wild type | ATCC 700392 |
| Cp90-84 | Clinical isolate | ETSUa Clinical Microbiology Laboratory |
| Cp20-84 | Clinical isolate | ETSU Clinical Microbiology Laboratory |
| Hp7-91 | Clinical isolate | ETSU Clinical Microbiology Laboratory |
| Hp64-93 | Clinical isolate | ETSU Clinical Microbiology Laboratory |
| Hsp110-93 | Clinical isolate | ETSU Clinical Microbiology Laboratory |
| 26695/hp0021::cm | 26695 with chloramphenicol resistance cassette in hp0021 | This work |
| E. coli strains | ||
| W3110 | Wild type; F− λ− | E. coli Genetic Stock Center (Yale University) |
| XL-1 Blue | recA1 endA1 gyrA96thi-1 hsdR17 supE44 relA1 lac [F′ proAB lac1qZΔM15::Tn10 (Tetr)] | Stratagene |
| NovaBlue(DE3) | endA1 hsdR17(rK12− mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac (DE3) [F′ proAB lac1qZΔM15:Tn10 (Tetr)] | Novagen |
| H. influenzae Rd | Wild type | ATCC 51907 |
| Plasmids | ||
| pET21a | Vector containing a T7 promoter; Ampr | Novagen |
| pWaaAEc | pET21a containing E. coli W3110 waaA | This work |
| pWaaAHi | pET21a containing H. influenzae waaA | This work |
| pWaaAHp1 | pET21a containing H. pylori J99 waaA | This work |
| pWaaAHp2 | pET21a containing H. pylori 26695 waaA gene | This work |
| pBluescript II SK (+) | Lac expression vector; Ampr | Stratagene |
| pBSHp0021 | pBluescript II SK (+) containing hp0021 | This work |
| pBSHp0021NX | pBSHp0021 with engineered NdeI and XhoI sites | This work |
| pBSHp0021::cm | pBSHp0021NX containing chloramphenicol resistance cassette in hp0021 | This work |
| pHel2 | E. coli-H. pylori shuttle vector; Camr | 36 |
a
ETSU, East Tennessee State University.