FIG. 5.
(A and B) Anaerobic growth in VM medium (A) and concomitant reduction of vanadate (B) for VRD1 (□), VRD2 (▴), MR-1R (▪), VRD1 (▿) amended with 50 μM vitamin K2, and VRD2 (▵) amended with 50 μM vitamin K2. (C and D) Growth of (C) and concomitant reduction of vanadate by (D) mutant VRD3 (♦) and VRD3/pEC86 (▵), compared to MR-1R (▪). (E and F) Growth of (E) and concomitant reduction of vanadate by (F) mutant VRD4 (▾), compared to MR-1R (▴). All measurements at each time were obtained by calculating the average values for at least three independent incubations.