Table 1.
Primary and secondary antibodies used and their dilutions.
| Antigen | Species | Dilution | Provider | Protocol Specification |
|---|---|---|---|---|
| HAT-1 | Rabbit monoclonal | 1:1000 | Abcam Cambridge, UK (ab193097) |
100% Triton 0.1% in PBS, 10 min, before incubation with blocking solution |
| IPO-4 | Rabbit monoclonal | 1:100 | Abcam (ab181046) |
10 mM sodium citrate pH = 6 before incubation with blocking solution |
| IPO-5 | Rabbit polyclonal | 1:100 | Abcam (ab187175) | 100% Triton 0.1% in PBS, 10 min, before incubation with blocking solution |
| NASP | Mouse monoclonal | 1:100 | Santa Cruz Biotechnology, Santa Cruz, CA, USA (sc.514669) | 10 mM sodium citrate pH = 6 before incubation with blocking solution |
| RBBP7 | Rabbit monoclonal | 1:2000 | Abcam (ab259957) | 10 mM sodium citrate pH = 6 before incubation with blocking solution |
| H3 | Rabbit polyclonal | 1:100 | Abcam (ab1791) | 100% Triton 0.1% in PBS, 10 min, before incubation with blocking solution |
| H4 | Rabbit monoclonal | 1:500 | Abcam (ab51997) | EDTA pH = 9 before incubation with blocking solution |
| ASF-1 | Rabbit polyclonal | 1:100 | Abcam (ab235358) | 100% Triton 0.1% in PBS, 10 min, before incubation with blocking solution |
| PGAM-1 | Rabbit monoclonal | 1:25 | Abcam (ab247037) | EDTA pH = 9 before incubation with blocking solution |
| AIF-1 | Goat polyclonal | 1:500 | Abcam (ab5076) | EDTA pH = 9 before incubation with blocking solution |
| IL-12A | Rabbit monoclonal | 1:100 | Abcam (ab131039) | EDTA pH = 9 before incubation with blocking solution |
| IL-18 | Rabbit monoclonal | 1:250 | Abcam (ab243091) | 10 mM sodium citrate pH = 6 before incubation with blocking solution |
| IL-10 | Rabbit Polyclonal | 1:100 | Abcam (ab217941) | 100% Triton 0.1% in PBS, 10 min, before incubation with blocking solution |