TABLE 2.
Identified changes in the proteomes of S. pyogenes microcolonies
| Functional category and identity | Function | Quantitationa
|
||
|---|---|---|---|---|
| M49-CS101 | Alt. 1 | Alt. 2 | ||
| Division | ||||
| Spy1514 | Cell division initiation protein (DivIVAS) | 0 ± 0 | 3,181 ± 2,004 | (0; 0; 854) |
| Spy1514 | Cell division initiation protein (DivIVAS) | 0 ± 0 | (289; 283; 2,176)b | (0; 0; 1,868) |
| Spy1514 | Cell division initiation protein (DivIVAS) | 6,442 ± 907 | 1,239 ± 721 | (0; 0; 926) |
| Spy1520 | Cell division protein (FtsZ) | 12,835 ± 650 | 5,301 ± 1,173 | 4,380 ± 1,760 |
| Metabolic | ||||
| Spy0731 | Enolase | 0 ± 0 | 1,588 ± 205 | (0; 0; 1,155) |
| Spy0731 | Enolase | 0 ± 0 | 437 ± 198 | (0; 802; 168) |
| Spy0731 | Enolase | 20,778 ± 1,599 | 47,082 ± 2,448 | 28,886 ± 23,407 |
| Spy0927 | Adenine phosphoribosyltransferase | 730 ± 175 | (0; 737; 0) | 0 ± 0 |
| Spy1128 | Phosphotransacetylase | 6,337 ± 217 | 3,557 ± 570 | (618; 0; 2,135) |
| Spy1150 | NADH oxidase (NOX) | 0 ± 0 | 585 ± 256 | 352 ± 14 |
| Spy1151 | l-lactate dehydrogenase | 3,619 ± 1,195 | (0; 3,988; 1,918) | 0 ± 0 |
| Spy1541 | Carbamate kinase | 1,351 ± 1,059 | (0; 559; 0) | 0 ± 0 |
| Spy1541 | Carbamate kinase | 16,592 ± 3,443 | 2,906 ± 508 | 1,874 ± 1,189 |
| Spy1618 | Cysteine synthase/O-acetylserine lyase | 158 ± 38 | 759 ± 182 | (0; 0; 579) |
| Spy1745 | Biotin carboxylase | 45 ± 64 | 444 ± 157 | (0; 0; 325) |
| Spy1881 | Phosphoglycerate kinase | 789 ± 231 | 1,576 ± 832 | 0 ± 0 |
| Transcription | ||||
| Spy0080 | DNA-directed RNA polymerase alpha subunit | 615 ± 304 | (0; 668; 605) | 0 ± 0 |
| Spy0611 | Translation elongation factor EF-Tu | 4,914 ± 201 | 11,520 ± 2,873 | 3,915 ± 2,908 |
| Spy0611 | Translation elongation factor EF-Tu | 1,809 ± 582 | (802; 1,034; 0) | 0 ± 0 |
| Spy0611 | Translation elongation factor EF-Tu | 0 ± 0 | 1,424 ± 257 | 0 ± 0 |
| Spy2093 | Elongation factor Ef-Ts | 201 ± 138 | (1,059; 995; 0) | 0 ± 0 |
| Spy2093 | Elongation factor Ef-Ts | 4,448 ± 2,762 | 1,383 ± 441 | 0 ± 0 |
| Miscellaneous | ||||
| M18_1302 | Hypothetical phage protein | 4,252 ± 1,692 | 247 ± 233 | 0 ± 0 |
| Spy0022 | Fatty acid/phospolipid synthesis | 558 ± 115 | 1,300 ± 123 | 1,548 ± 844 |
| Spy0022 | Fatty acid/phospolipid synthesis | 42 ± 60 | 363 ± 111 | (0; 0; 159) |
| Spy0022 | Fatty acid/phospolipid synthesis | 1,959 ± 1,713 | (1,080; 2,064; 0) | 0 ± 0 |
| Spy0123 | Heat shock protein HSP33 | 9,700 ± 1,383 | 3,520 ± 726 | 4,628 ± 2,996 |
| Spy0145 | Conserved hypothetical protein | 2,188 ± 65 | 0 ± 0 | 0 ± 0 |
| Spy0416 | Cell envelope proteinase | 12,023 ± 3,354 | 78 ± 134 | 9,932 ± 8,623 |
| Spy1527 | GTP binding protein TypA/BipA | 2,523 ± 259 | 3,184 ± 179 | 1,300 ± 1,216 |
| Spy1760 | Heat shock protein 70 | 1,429 ± 1,164 | 32 ± 28 | 0 ± 0 |
| Spy1896 | Trigger factor (prolyl isomerase) | 2,318 ± 69 | 2,395 ± 1,558 | (0; 1,257; 0) |
| Spy2010 | C5a peptidase precursor | 379 ± 212 | 32 ± 55 | 0 ± 0 |
Quantitative values for protein spots are in pixels per spot area from two independent experiments for M49-CS101 and three independent experiments for Alt. 1 and Alt. 2. The values are given as a mean ± standard deviation for M49-CS101, Alt. 1, and Alt. 2 if the values obtained were consistent across all replicates. If the spot quantitation was inconsistent across the three replicates for either Alt. 1 or Alt. 2, individual values in pixels per spot area are given for the three independent replicates. These are considered lower-confidence values.
These data are given as three individual data points, rather than a mean ± standard deviation, for clarity. Since these Alt. 1 data points are consistently elevated above what is observed for M49-CS101, this spot is considered a high-confidence increase.