FIG. 2.

Quantitative RT-PCR assessment of transcriptional heat shock induction in D. radiodurans R1 wild-type (WT) compared to sig1 mutant cells. (A) hsp20 and dnaK 1 h after induction to heat shock at 48°C. (B) DR1314 and groES expression 5 min after heat shock. RNA was harvested from wild-type and sig1 cells following shift to heat shock at 48°C for 5 min (B) or 1 h (A); 30°C cultures served as a control. Following cDNA synthesis, QPCR was performed using primers that anneal to 100- to 200-bp sequences internal to the gene of interest. Data were quantified using a relative scheme in which relative expression values greater than 1 indicate heat shock induction. Data shown represent the averages ± standard deviations of at least three biological replicate experiments.