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. 2024 May 15;14(10):1011. doi: 10.3390/diagnostics14101011

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunoexpression of Td-dentilisin. Gingival tissue specimens were graded and scored as (A) negative (0), (B) very low-positive (0.5), (C) low-positive (1), (D) moderate-positive (2), and (E) strong-positive (3). The gingival tissue specimens (A,B) represent periodontitis classification stage I /grade A, specimens (B,C) stages I–II grade B, and specimens (D) stages III–IV/grade C, respectively. The chromogen (red) was 3-amino-9-ethylcarbazole (AEC), and the counterstain was hematoxylin. Td-dentilisin was detected in all red-stained regions on each tissue segment. Magnification 200×.