Table 1.
Immunoexpression of Td-dentilisin, MMP-8, and RNA expressions of MMP-8 and its regulators MMP-7, MMP-2, TIMP-1, and IL-1β in CP-stages III/IV grades B/C gingivae vs. HC gingivae. In IHC, n = 10 for HC, and n = 9 for CP, and in RNA sequencing/transcriptomics, n = 20 for HC, and n = 18 for CP.
| Parameters | Healthy (HC) | Periodontitis (CP) | Significance (p-Value) |
|---|---|---|---|
| Immunohistochemical staining of gingival tissue | |||
| Td-dentilisin | 1.00 ± 0.21 | 2.44 ± 0.06 | *, p = 0.0038 |
| MMP-8 | 1.00 ± 0.20 | 4.33 ± 0.41 | *, p = 0.0001 |
| RNA sequencing/transcriptomics | |||
| MMP-8 | 0.35 ± 0.62 | 0.46 ± 0.84 | - |
| MMP-7 | 1.78 ± 4.17 | 49.92 ± 106.16 | *, p = 0.0280 |
| MMP-2 | 191.27 ± 138.23 | 147.05 ± 138.66 | - |
| TIMP-1 | 47.28 ± 16.62 | 66.01 ± 49.47 | - |
| IL-1β | 14.73 ± 16.78 | 71.47 ± 61.32 | *, p = 0.0001 |
| GAPDH | 2491.42 ± 630.29 | 2596.36 ± 840.13 | |
* = significantly different, p < 0.05, t-test. Abbreviations: MMP-8 = matrix metalloproteinase 8; MMP-7 = matrix metalloproteinase 7; MMP-2 = matrix metalloproteinase 2; TIMP-1 = tissue inhibitor matrix metalloproteinase 1; IL-1β = interleukin 1 beta; GAPDH = glyceraldehyde 3-phosphate-dehydrogenase; HC = healthy control; CP = chronic periodontitis.