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(A) Western blot analysis of CrgA expression in the complementing strain (ΔRCrgA-C) grown with increasing amounts of IPTG. Lanes 1 to 4 contained total lysates from strains grown in the presence of 0, 10, 30, and 100 μM IPTG, respectively. Total RNA was extracted from samples of the same cultures and used in primer extensions to monitor accumulation of RNA synthesized from the PcrgA (B) and PmdaB (C) promoters.
