TABLE 2.
Primers used in this study
| Olignucleotide | Sequencea | Siteb |
|---|---|---|
| adk-PE | CGCGCCTAAAAGTAATGC | |
| Com1 | attcagcccgggGCGTGGCTGATCAAACGCACCG | XmaI |
| Com2 | gttatgcatAGGTCTCGAATTGTGGATCCCACGC | NsiI-BamHI |
| Com3 | ggatccACAATTCGAGACCTATGCATTAACATAG | NsiI-BamHI |
| Com4 | attcagactagtAATGCCGCCTCCGTCGGTTTGC | SpeI |
| crdw-L | ccgggaattcTTAGGCTACGGCATCTGCGCC | EcoRI |
| crdw-R | attcgcggatccGTCGGCGGCAATGGCAAT | BamHI |
| CrgA-N | attcgcatATGAAAACCAATTCAGAAGAACTGACC | NdeI |
| Crg-R | attcgatgcatAGAATTATCCACAGAGATTGTTTTC | NsiI |
| crup-L2 | attcgcggatcCTTGAACAAATACGGTCAG | BamHI |
| crup-R2 | aactgcagCATAGCCGGCATCAATCACGG | PstI |
| Ery-DO-C | CAGGTTACTAAAGGGAATGGAG | |
| Eryt-DO | CCGTAGGCGCTcGaGACCTCTTTAGCTTCTTG | XhoI |
| Eryt-UP | GCAAACTgcAGAGTGTGTTGATAG | PstI |
| Fla-DO-C2 | CTCGAAACCGGTTCTGACGG | |
| Fla-DO-L | ATAAATGTAAAGGaTCCGTTTCATAGCTAAGG | BamHI |
| Fla-DO-R | CGCCGTCAACCCgGgTGCCGAGCTGGAAAAAGAGC | XmaI |
| Fla-UP-C | CTGAAGCAAAGTCGGAAAACGCCGGC | |
| Fla-UP-L | GGTTCCGTACTAgTTGTACTGTCTGC | SpeI |
| Fla-UP-R | aatttaactcgagCCACCAATCCCACACCACCCTTACC | XhoI |
| LAC-DO-C | CGCTACCATTACCAGTTGGTCTGG | |
| LAC | CTTGTTGGTCAAAGTAAACGACAT | |
| mdaB-PE | CCGTGAGAATGTCCGAACGC | |
| MDER-PE1 | GATACTGCACTATCAACACA | |
| NMB1856L | ATTGCGCGCCTTCTTCCGTC | |
| NMB1856R | CATAGCCGGCATCAATCACG | |
| Pcrg1L | atatatgcatgcTCTGAATTGGTTTTTATCGTGTTTCC | SphI |
| Pcrg1R-N | atatatatgcatTTGTTACCTCGTTTGTGAATTGATG | NsiI |
| Pcrg2L | atatatgcatgcCTGATTATTTCATTTGACGCAAAAG | SphI |
| Pcrg2R-N | atatatatgcatCTTTATAATTTAAAAGTGCAAAAATAAG | NsiI |
| Pcrg3R-N | atatatatgcatTTAAAAGTGCAAAAATAAGAAAACA | NsiI |
| Pcrg4R-N | atatatatgcatATGTTCCAACACACGGGATGGCAC | NsiI |
| pilC-PE2 | TTTTAAAGTTTTATTCATCG | |
| pilE-PE1 | GAAGGGTGTTCATAAAATTAC | |
| Pind-F | attcgggatccGCGTTGCGCTCACTGCCCGC | BamHI |
| Pind-R | aatgcatgcatggtcatatgTGTTTCCTGTGTGAAATTG | NsiI-NdeI |
| sia-PE1 | ACCTGTAATGCAAAGAATTC |
a
Uppercase letters in Roman type indicate N. meningitidis-derived sequences, italicized uppercase letters indicate E. coli-derived sequences, lowercase letters indicate sequences added for cloning purposes, and underlined letters indicate recognition sites.
b
Restriction enzyme sites added for cloning purposes.