TABLE 1.
Strains and plasmids used in this studya
| Strain or plasmid | Serotype | Characteristic(s) | Source or reference |
|---|---|---|---|
| Strains | |||
| Y. pseudotuberculosis | |||
| IP32637 | I | Wild type (HPI+) | IP collection |
| IP32637/do | I | irp2::kan Rifr used as donor strain | This study |
| IP32637/do Δhef | I | hef::kan Rifr | 22 |
| IP32637/do(pBAD33-hef) | I | IP32637/do harboring pBAD33-hef | 22 |
| IP32637/do Δint | I | int::kan Rifr | 22 |
| IP32637/do ΔrecA | I | recA::spe | This study |
| IP32637/do Δor1188 | I | or1188::tmp | This study |
| IP32637/re | I | ΔHPI Nalr used as recipient strain | 22 |
| IP32637/re HPI+ | I | HPI+ Nalr | This study |
| IP32637/re ΔrecA | I | recA::spe | This study |
| IP32533 | I | Wild type (HPI+) | IP collection |
| IP32533/re | I | ΔHPI Nalr | 22 |
| IP32781 | I | Wild type (HPI+) | IP collection |
| IP32781/re | I | ΔHPI Nalr | 22 |
| IP32953 | I | Wild type (HPI+) | IP collection |
| IP32953/re | I | Nalr | This study |
| IP33306 | II | Wild type (HPI−) | IP collection |
| IP33306/re | II | Nalr | This study |
| IP31830 | IV | Wild type (HPI−) | IP collection |
| IP31830/re | IV | Nalr | This study |
| Y. enterocolitica | |||
| IP845 | O:20 | Wild type (HPI+) | IP collection |
| IP845/re | O:20 | Nalr | This study |
| IP11666 | O:16 | Wild type (HPI−) | IP collection |
| IP11666/re | O:16 | Nalr | This study |
| Y. pestis | |||
| 6/69 | NA | Wild type (HPI+) | IP collection |
| 6/69/re | NA | Nalr | This study |
| Plasmids | |||
| pUC4K | High copy number, Kmr | Amersham | |
| pSW25 | oriT ccdB Sper | 11 | |
| pBAD33-hef | hef cloned under the pArab promoter, low copy number, Cmr | 22 | |
| pKOBEGsacB | Ts, low copy number, sacB Cmr | 12 | |
| pGP704N | Suicide vector, Ampr | 27 | |
| pNot::Ptac-dfrB1 | Tmpr | 5 | |
| pGP704N-dfr | dfr amplified from pNot::Ptac-dfrB1, Tmpr Ampr | This study |
a
IP, Institut Pasteur; NA, not applicable.