FIG. 4.

Effect of SDS treatment on WT and TCP⁻ mutant biofilms on a squid pen. Three-dimensional side views, generated by confocal scanning laser microscopy, of SDS-treated (+ SDS) or untreated (− SDS) biofilms of the WT and TCP mutant strains of V. cholerae showing the detachment of most cells from the undifferentiated TCP⁻ biofilm after SDS treatment are presented; the structured WT biofilms remained unaffected. The substratum (squid pen) is located at the bottom of the images. Bars, 50 μm.