TABLE 3.
Incompatibility assays
| parS site on resident target plasmida | Par protein(s) suppliedb | Supernumerary parS sites carried by incoming pACYC184 derivativec | % Retention of target plasmid (25 generations) |
|---|---|---|---|
| P7 | P7 | P7 | 3 |
| P1 | P1 | P1 | 3 |
| pWR100 | pWR100 | pWR100 | 5 |
| P1 | P1 | pWR100 | 10 |
| P7 | P7 | pWR100 | 95 |
| pWR100 | pWR100 | P1 | 5 |
| pWR100 | pWR100 | P7 | 60 |
| P1 | P1 | P7 | 95 |
| P7 | P7 | P1 | 95 |
a
The target plasmids were λ-P1::5RΔ1005::pALA1993 (P7parS), λ-P1::5RΔ1005::pALA1952 (P1parS), or λ-P1::5RΔ1005::pALA2602 (pWR100parS).
b
The Par proteins were supplied from pALA1414 (P7 ParA and ParB), pALA1413 (P1 ParA and ParB), or pALA2620 (pMT1 ParA and ParB).
c
Supernumerary parS sites were provided by the presence of the following pACYC184 derivatives: pALA1850 (P7parS), pALA1849 (P1parS), or pALA2609 (pWR100parS).