TABLE 4.
Effect of deleting the noncanonical 21-bp sequence from the pWR100parS site
| Plasmid | par operon carried | Test plasmid parSa site | % Retentionb (25 generations) |
|---|---|---|---|
| pGB2 | None | pWR100parSΔ21 | <2 |
| pALA2620 | pWR100parA-parB | pWR100parS | 72 |
| pALA1413 | P1parA-parB | pWR100parS | 96 |
| pGB2 | None | pWR100parSΔ21 | <2 |
| pALA2620 | pWR100parA-parB | pWR100parSΔ21 | 28 |
| pALA1413 | P1parA-parB | pWR100parSΔ21 | 78 |
a
The test plasmids were λ-P1::5RΔ1005::pALA2602 (pWR100parS) or λ-P1::5RΔ1005::pALA2621 (pWR100parSΔ21).
b
All values were obtained from a single experiment. Duplicate experiments gave similar results. The controls using the wild-type pWR100parS are duplications of tests reported in Table 1, where similar but not identical values were obtained.