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. 2005 May;187(10):3511–3520. doi: 10.1128/JB.187.10.3511-3520.2005

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FIG.3. — Localization of GFP-tagged derivatives of SpoIIE mutants during vegetative growth. SB220 (wild-type SpoIIE) (A), KC506 (MalF-SpoIIE) (B), KC507 (SpoIIE^Q483A) (C), SB414 (SpoIIE^S361F) (D), KC552 (SpoIIE^D686A) (E), SB221 (SpoIIE^ΔregII) (F), and KC542 (SpoIIE^ΔregI) (G) were grown in hydrolyzed casein medium supplemented with 0.5 mM IPTG for 3 h (see Materials and Methods), and the GFP (left panels) and membrane stain (middle panels) were visualized by fluorescence microscopy. Right panels are overlays of GFP (green) and membrane stain (red). Arrowheads in panel G mark punctate spots of fluorescence that may indicate spiral-like structures.