TABLE 1.

Plasmids and H. pylori strains used in this study

Plasmid or strain	Relevant characteristics	Source or reference
Plasmid
pVacAKm	vacA::aphA in pGEMT-Easy	33
pRecAKm	recA::aphA in pGEMT-Easy	33
pMutS2	HP0621 in pGEMT-Easy	This work
pMutS2Km	mutS2::aphA in pGEMT-Easy	This work
pVacA0	vacA with 0-bp deletion cassette	6
pVacA50	vacA with 50-bp deletion cassette	6
pVacA100	vacA with 100-bp deletion cassette	6
pMutS2-0	mutS2 with 0-bp deletion cassette	This work
pMutS25-0	mutS2 with 50-bp deletion cassette	This work
pMutS2-100	mutS2 with 100-bp deletion cassette	This work
pAD1	ureAB fragment in pUC18	4
pADC-MutS2	pAD1 with mutS2 and CAT cassette	This work
pRpsLWT	800-bp rpsL fragment in pGEM3-ZF	This work
pRpsLSS	800-bp rpsL fragment (A128G) in pGEM3-ZF	This work
pAD1-Cat	pAD1 with CAT cassette	This work
H. pylori strain
JP26	Wild-type strain	33
JP26/vacA::aphA	ΔvacA with aphA insertion	33
JP26/recA::aphA	ΔrecA with aphA insertion	33
JP26/mutS2::aphA	ΔmutS2 with aphA insertion	This work
JP26 mutS2comp	mutS2::aphA complemented with mutS2 in ureAB locus	This work
JP26/vacA::0	vacA interrupted with 0-bp deletion cassette	6
JP26/vacA::50	vacA interrupted with 50-bp deletion cassette	6
JP26/vacA::100	vacA interrupted with 100-bp deletion cassette	6
JP26/mutS2::0	mutS2 interrupted with 0-bp deletion cassette	This work
JP26/mutS2::50	mutS2 interrupted with 50-bp deletion cassette	This work
JP26/mutS2:100	mutS2 interrupted with 100-bp deletion cassette	This work