TABLE 2.
Oligonucleotide primers used in this study
| Primer designation | Genomic locationa | Primer sequence (5′ → 3′)b |
|---|---|---|
| MutS5 | 668833-668855 | AAGAGATCGCTTTGCAAAGAGC |
| MutS3 | 666264-666287 | GCTAAAGATTTTAAGGGTTTTGG |
| MutinvF1 | 667275-667296 | CGGGATCCTGAAGAAAAAGAACGGCCCAC |
| MutinvR1 | 667337-667354 | CGGGATCCCTGCCATTAACACGCTCAAGC |
| MutSXbaI | 668698-668679 | GCTCTAGATGTCAGACGCTCCAAAAAG |
| MutSSmaI | 666281-666296 | TCCCCCGGGTTTTGGCTCAATAGC |
a
Location based on the sequence of strain 26695 (68).
b
Restriction sites underlined; EcoRI (GGATCC), XbaI (TCTAGA), and SmaI (CCCGGG).