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. 2005 May;187(10):3486–3495. doi: 10.1128/JB.187.10.3486-3495.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 3. — Effects of i2 mutations on VirB9 partner interactions with VirB7 and VirB10. (A) Detection of disulfide-cross-linked VirB7-VirB9 dimers (∼36-kDa) and VirB9 monomers (29-kDa) in total cell extracts by nonreducing SDS-PAGE and immunoblot analysis with anti-VirB9 antibodies. (B) Detection of the putative VirB9-VirB10 complexes by immunoprecipitation with anti-VirB10 antibodies. Immunoprecipitates were analyzed for VirB9 and VirB10 by SDS-PAGE and development of immunoblots with antibodies to both proteins. WT, strain A348; ΔB9, strains PC1009 and PC1009 producing native VirB9 (B9) and the VirB9.i2 mutants (i2 insertion sites shown).