TABLE 1.
Uncoupling mutations do not bypass VirB2 pilin requirement for DNA substrate translocation
| Host strain | Complementing construct | Virulence |
|---|---|---|
| A348 (WT) | +++ | |
| ΔvirB9 | − | |
| ΔvirB9 | PvirB-virB9 | ++ |
| ΔvirB11 | PvirB-virB11 | ++ |
| ΔvirB9 | PvirB-virB9.i2-116 | ++ |
| ΔvirB9 | PvirB-virB9.i2-236 | ++ |
| ΔvirB9 | PvirB-virB9.i2-286 | ++ |
| ΔvirB11 | PvirB-virB11-I265T | ++ |
| ΔvirB2ΔvirB9 | PvirB-virB2, PvirB-virB9 | ++ |
| ΔvirB2ΔvirB11 | PvirB-virB2, PvirB-virB11 | ++ |
| ΔvirB2ΔvirB9 | PvirB-virB9.i2-116 | − |
| ΔvirB2ΔvirB9 | PvirB-virB9.i2-236 | − |
| ΔvirB2ΔvirB9 | PvirB-virB9.i2-286 | − |
| ΔvirB2ΔvirB11 | PvirB-virB11-I265T | − |