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. 2005 May;187(10):3572–3580. doi: 10.1128/JB.187.10.3572-3580.2005

FIG. 3.

FIG. 3.

FIG. 3.

Localization of ParB-EGFP foci in aerial hyphae of J3310. Numbered arrows refer to developmental stages shown in panel C. A. Images of ParB-EGFP fluorescence (top row), DNA stained with 7-AAD (bottom row), and an overlay of the two fluorescence signals. B. Images of ParB-EGFP fluorescence (top row), cell walls stained with WGA conjugate (bottom row), and an overlay of the two fluorescence signals. C. Diagram showing sequential stages of aerial hyphal development with respect to ParB-EGFP focus formation (see Results) (red, cell wall; green, ParB; blue, DNA). Percentages show the frequencies of occurrence of hyphae in the particular stage (white number, average; blue number, determined in DNA-stained images; red number, determined in cell wall-stained images). D. Gaussian distribution of distances measured between ParB-EGFP complexes in arrays of ParB-EGFP foci. Empty circles and thin line, measurement for hyphae in developmental stage 2 (n = 100); black squares and thick line, measurement obtained for hyphae containing septa (n = 104).