FIG. 2.

Multiple alignment of Bacillus subtilis (Bsubt) YwqE and its homologues from different gram-positive bacteria, as follows: Bacillus cereus (Bcere), Oceanobacillus iheyensis (Oihen), Lactococcus lactis (Llact), Streptococcus suis (Ssuis), Streptococcus pneumoniae (Spneu), and Streptococcus thermophilus (Sther). The PHP phosphoesterase from Yersinia pestis (YpestPHP) was added as the outgroup. Residues conserved in both PHP phosphoesterases and YwqE homologues are highlighted. Residues mutated in this study are designated by arrows.