TABLE 1.
Effects of phosphatase inhibitors on YwqE activity
| Phosphatase inhibitor(concn) or condition | % Relative activity ± SEa |
|---|---|
| No inhibitor | 100.0 ± 0.5 |
| Iodoacetamide (1 mM) | 100.9 ± 0.6 |
| NEM (1 mM) | 137.7 ± 0.6 |
| Na3VO4 (1 mM) | 11.4 ± 5.1 |
| NaF (1 mM) | 85.0 ± 1.2 |
| NaPP (1 mM) | 31.2 ± 1.6 |
a
Inhibitors were added to the reaction buffer at a final concentration of 1 mM. Iodoacetamide and NEM were preincubated with the enzyme for 10 min on ice. The assay mixture contained 300 nM enzyme and 3 mM of pNPP as the substrate. The buffer used was 50 mM HEPES, pH 8.0, and 1 mM MnCl2.