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. 2005 Jun;187(11):3671–3677. doi: 10.1128/JB.187.11.3671-3677.2005

FIG. 5.

FIG. 5.

Divalent metal ion dependence for YpdF and YpdE. (a) Divalent metal ion requirement for YpdF. The substrate used was Met-Ala-Ser. Metal ions included Co2+ (lane 4), Cu2+ (lane 5), Zn2+ (lane 6), Ni2+ (lane 7), Mg2+ (lane 8), Ca2+ (lane 9), Mn2+ (lane 10), and Fe2+ (lane 11). Other lanes are as follows: lane 1, MAS only; lane 2, AS only; lane 3, methionine only; lane 12, MAS with no metal ions but YpdF; lane 13, MAS with no enzyme but Co2+. (b) Divalent metal ion requirement for YpdE. The substrate used was Met-Ala-Ser. Metal ions included Co2+ (lane 5), Mn2+ (lane 6), Cu2+ (lane 7), Fe2+ (lane 8), Ca2+ (lane 9), Ni2+ (lane 10), Zn2+ (lane 11), and Mg2+ (lane 12). Other lanes are as follows: lane 1, MAS only; lane 2, alanine only; lane 3, methionine only; lane 4, MAS with YpdE but no metal ion.