TABLE 2.
Oligpeptides used in this study
| Enzymea and test result | Peptide(s)b |
|---|---|
| YpdF | |
| + | Met-Ala-Ser, Met-Pro-Gly, Met-Ser-Gly |
| + | Met-Pro-Gly-Ala-Arg-Ala-Asp-Ala-Ala-Leu-Ser-Met-Ala-Asp-Ala |
| + | Ala-Pro-Ala |
| + | Asn-Pro-Thr-Asn-Leu-His |
| − | Met-Arg-Phe, Met-Gly-Gly, Met-Gly-Met, Met-Met-Ala, Met-Leu-Gly, Met-Phe-Gly, For-Met-Ala-Ser, Ac-Met-Ala-Ser |
| − | Met-Cys-Gly-Lys |
| − | Met-Lys-Arg-Ser-Arg-Gly-Pro-Ser-Pro-Arg-Arg |
| − | Ala-Gly-Ser-Glu |
| − | Ala-Ala-Ala |
| − | Ser-Ser-Ser |
| − | Arg-Gly-Asp-Ser-Pro-Ala-Ser-Lys-Lys-Pro |
| − | Leu-Asp-Leu-Leu-Phe-Leu |
| − | Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu |
| − | Gly-Pro-Ile-Ser |
| − | Arg-Pro-Lys-Pro-Gln-Gln-Phe |
| − | Leu-Pro-Pro-Ser-Arg |
| − | Val-Pro-Leu |
| − | Ser-Pro-Val-Thr-Leu-Asp-Leu-Arg-Tyr |
| − | Tyr-Pro-Ile-Ser-Leu |
| − | Asp-Pro-Ala-Phe-Asn-Ser-Trp-Gly-NH2 |
| YpdE | |
| + | Met-▾Ala-▾Ser |
| + | Lys-▾Ala-Arg-Val-Nle-p-nitro-Phe-Glu-Ala-Nle-NH2 |
| + | Gln-▾Ala-▾Thr-▾Val-▾Gly-▾Asp-▾Val-▾Asn-▾Thr-▾Asp-▾Arg-Pro-Gly-Leu-Leu-Asp-Leu-Lys |
| + | Met-▾Ala-▾Gly-Pro-His-Pro-Val-Ile-Val-Ile-Thr-Gly-Pro-His-Glu-Glu |
| + | Asp-▾Ala-▾Glu-▾Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu |
| + | Tyr-▾Leu-▾Leu-Pro-Ala-Glu-Val-Asn-Ile-Asp |
| − | Ac-Ala-Ala-Ala, Ac-Met-Ala-Ser, Ac-Pro-Leu-Gly |
| − | Ac-Lys-D-Ala-D-Ala, Ac-Asp-Arg-Gly-Asp-Ser, For-Met-Ala-Ser |
| − | His-Lys-Ala-Arg-Val-Leu-p-nitro-Phe-Glu-Ala-Ser-NH2 |
| − | Trp-Met-Asn-Ser-Thr-Gly-Phe-Thr-Lys-Val-Cys-Gly-Ala-Pro-Pro-Cys |
| − | Pro-Ala-Glu-Asp-Leu-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr-NH2 |
| − | Ac-Ala-Ala-Asp-Ile-Ser-Gln-Trp-Ala-Gly-Pro-Leu |
| − | Ac-Ala-Thr-Leu-Asp-Ala-Leu-Leu-Ala-Leu-Arg-Arg-Ile-Gln-NH2 |
| − | Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala |
a
A plus sign means that the enzyme shows aminopeptidase activity on this substrate by TLC assay or MALDI-TOF experiments; a minus sign means that no cleavage was observed.
b
The symbol ▾ marks the site where the cleavage can be confirmed by MALDI-TOF spectrums. Ac stands for acetyl group, and For stands for formyl group.