TABLE 2.
Sequences of the primers used in this study
| Primer | Sequence (5′→3′) | Location |
|---|---|---|
| lacR1 | GACTACTATTTGGAAAGCGC | yjiD |
| lacR2 | TGATCTATATCGACGCTTAGG | fruR |
| lacR3 | TTAATTGTCAAAGCCGTTAGC | fruR |
| lacR4 | GTGTAAGTTCTGCACCACC | fruR |
| lacC1 | CACTAGGCTTTCTAGGTGG | fruC |
| lacC2 | GGTCGAATCACCAGCACC | fruC |
| lacC3 | AAGTTTTGGTCCAGCCGC | fruC |
| lacC4 | GAAGTAGTCAAGTGCTGGG | fruC |
| fruA1 | AAGCCTTGGAAATGGGCG | fruA |
| fruA2 | GTATTGATTGCTGCCATTGGG | fruA |
| M1 | TGAGTTTTTTTTGGAAGAAAAT GATAGTTAATG | fru promoter region |
| M2 | TGGAAGAAAATGATAGTTAATG | fru promoter region |
| M3 | TGATTGATTTTGAAAGATAAAG | fru promoter region |
| M4 | AAGAAGAAAAAAATAGTTAAAAA TTGATTTAAAAAGATAAAGGa | fru promoter region |
| tuf1 | CGCGAACGTGGTATCACA | tuf |
| tuf2 | GTCCATTTGGGCAGCACC | tuf |
a
Inserted mutations are underlined.