TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| P. aeruginosa strains | ||
| PAO1 | Wild-type prototroph | B. Holloway |
| PAO1exsA | exsA mutant of PAO1, Cbr | 19 |
| PAO1pscC | pscC mutant of PAO1, Cbr | 19 |
| PAO1R | lasR mutant of PAO1 | 13 |
| PDO100 | rhlI mutant of PAO1, Hgr | 2 |
| PA103 | Cytotoxic respiratory clinical isolate, Fla− | 7 |
| E. coli strains | ||
| TOP10 F′ | φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 (F′ lacIq Tn10 mcrA Δ(mrr-hsdRMS-mcrBC) | Invitrogen |
| TG1 | supE Δ(lac-proAB) thi hsdRΔ5 (F′ traΔ36 proA+B+ZΔM15) | Laboratory collection |
| Plasmids | ||
| pRK2013 | Tra+ Mob+ Kmr | Laboratory collection |
| pCR2.1 | TA cloning vector, Apr | Invitrogen |
| pMP220 | Broad-host-range lacZ transcriptional fusion, Tcr | Laboratory collection |
| pMMB190 | Broad host range, tac promoter, Apr | Laboratory collection |
| pSBC6 | 1.2-kb DNA fragment carrying exsA, cloned in pMMB190 | This work |
| pSB307 | Broad host range, 275-bp-containing pS, pS-lacZ reporter, Tcr | This work |
| pSB302 | Broad host range, 211-bp-containing pT, pT-lacZ reporter, Tcr | This work |
| pSB303 | Broad host range, 191-bp-containing pY, pY-lacZ reporter, Tcr | This work |
| pSB305 | Broad host range, 219-bp-containing pD, pD-lacZ reporter, Tcr | This work |
| pSB308 | Broad host range, 320-bp-containing pG, pG-lacZ reporter, Tcr | This work |
| pSB313 | Broad host range, 552-bp-containing pN, pN-lacZ reporter, Tcr | This work |
| pPP4 | Broad host range, 249-bp-containing pC, pC-lacZ reporter, Tcr | This work |
| pBAD/Myc-HisA | Expression vector, araBAD promoter, addition of a poly(His) tag at the C-terminal part of the protein | Invitrogen |
| pPP8 | 1,145-bp DNA fragment (SBO25-26) containing exoY from PAO1, digested with PstI and HindIII, cloned in pBAD/Myc-HisA digested with PstI and HindIII | This work |
| pSBC4 | 270-bp DNA fragment (SBO9-10) containing pscF from PAO1, digested with NcoI and HindIII, cloned in pBAD/Myc-HisA digested with NcoI and HindIII | This work |