Alcaligin concentrations in cell-free culture supernatant fluids. Alcaligin concentrations in cell-free culture supernatant fluids after 24 h of growth in iron-depleted SS, expressed as alcaligin monomer concentrations (means ± standard deviations; n = 3), were measured by the quantitative CAS method. The concentrations shown were normalized to growth yields based on CFU/ml determinations. Abbreviations for strains used are as follows: wt, B013N; alcS, BRM16; alcS/alcSBb+, BRM16 (pRK/alcSBb); alcS/alcSBp+, BRM16 (pRK/alcSBp).